Co-team leaders : Harmen Reyngoudt & Benjamin Marty

Nuclear magnetic resonance (NMR) imaging and spectroscopy are powerful techniques that can be used to study muscle anatomy, physiology and biochemistry during one single examination.

In addition, muscle intrinsic contrast with NMR is incomparably richer than with other techniques and optimized radiofrequency pulse sequences allow quantifying specifically different variables, like the intramuscular fat fraction, muscle water relaxation times, muscle perfusion and oxygenation, which can be altered in patients with neuromuscular disorders.

Our laboratory focuses most of its efforts in the development of these quantitative NMR methods, their validation, their promotion and their implementation in mono-centric and multi-centric clinical trials related to neuromuscular disorder. These trials allow studying both the natural history of the pathology or the effect of a novel therapeutic approach.

More precisely, our research projects aim at:

• Developing novel NMR contrasts in preclinical and clinical settings aiming at detecting and quantifying different pathophysiological processes involved in the onset and progression of myopathies, like muscle inflammation, necrosis and fibrosis.
• Determining the prognostic value of these novel NMR variables on disease progression in several neuromuscular disorders
• Developing tools to investigate the muscle at exercise (non-magnetic ergometers, multinuclear interleaved data acquisitions, …).
• Accelerating NMR data acquisition using novel acquisition schemes (undersampled radial encoding) and multi-parametric data acquisitions (MR-fingerprinting, deep learning approaches, …).

Team members

Benjamin Marty, PhD, Co-team leader, AIM
Harmen Reyngoudt, PhD, Co-team leader, AIM
Jean-Marc Boisserie, AIM
Ericky Caldas de Almeida Araújo, PhD, AIM
Julien Dousseau, AIM
Yves Fromes, MD, PhD, AIM
Eric Giacomini, CEA, AIM
Julien Le Louër, AIM
Alfredo Lopez Kolkovsky, PhD, AIM
Béatrice Matot, PhD, AIM

Contact

+ 33 1 42 16 58 98
rdv_rmn@institut-myologie.org

Key publications

• B. Marty and P.G. Carlier: MR fingerprinting for water T1 and fat fraction quantification in fat infiltrated skeletal muscles. Magnetic Resonance in Medicine, 2020 Feb;83(2):621-634. doi: 10.1002/mrm.27960.
• T. Gidaro, H. Reyngoudt, J. Le Louër, A. Behin, F. Toumi, M. Villeret, E.C.A. Araujo, PY. Baudin, B. Marty, M. Annoussamy, J.Y. Hogrel, P.G. Carlier, L. Servais. Quantitative nuclear magnetic resonance imaging detects subclinical changes over 1 year in skeletal muscle of GNE myopathy. J Neurol. 2019 Oct 15. doi: 10.1007/s00415-019-09569-6.
• H. Reyngoudt, A.L. Lopez Kolkovsky, P.G. Carlier. Free intramuscular Mg2+ concentration calculated using both 31 P and 1 H NMRS-based pH in the skeletal muscle of Duchenne muscular dystrophy patients. NMR Biomed. 2019; Jun 11:e4115. doi: 10.1002/nbm.4115.
• B. Marty, P.G. Carlier. Physiological and pathological skeletal muscle T1 changes quantified using a fast inversion-recovery radial NMR imaging sequence. Sci Rep. 2019 May 2; 9(1):6852. doi: 10.1038/s41598-019-43398-x.
• T. Gerhalter, L.V. Gast, B. Marty, J. Martin, R. Trollmann, S. Schüssler, F. Roemer, F.B. Laun , M. Uder, R. Schröder, P.G. Carlier, A.M. Nagel: 23Na MRI Depicts Early Changes in Ion Homeostasis in Skeletal Muscle Tissue of Patients With Duchenne Muscular Dystrophy. Journal of Magnetic Resonance Imaging, 2019, doi: 10.1002/jmri.26681.
• G.J. Strijkers, E.C.A. Araujo, N. Azzabou, D. Bendahan, A. Blamire, J. Burakiewicz, P.G. Carlier, B. Damon, X. Deligianni, M. Froeling, A. Heerschap, K.G. Hollingsworth, M.T Hooijmans, D.C. Karampinos, G. Loudos, G. Madelin, B. Marty, A.M. Nagel, A.J. Nederveen, J.L. Nelissen, F. Santini, O. Scheidegger, F. Schick, C. Sinclair, R. Sinkus , P.L. de Sousa, V. Straub, G. Walter, H.E. Kan: Exploration of New Contrasts, Targets, and MR Imaging and Spectroscopy Techniques for Neuromuscular Disease – A Workshop Report of Working Group 3 of the Biomedicine and Molecular Biosciences COST Action BM1304 MYO-MRI. Journal of Neuromuscular Diseases, 2019;6(1):1-30.
• B. Marty, R. Gilles, M. Toussaint, A. Béhin, T. Stojkovic, B. Eymard, P.G. Carlier and K. Wahbi: Comprehensive Evaluation of Structural and Functional Myocardial Impairments in Becker Muscular Dystrophy using Quantitative Cardiac Magnetic Resonance Imaging. European Heart Journal: Cardiovascular Imaging, 2019, 20(8):906-915.
• B. Marty, B. Coppa and P.G. Carlier: Fast, precise, and accurate myocardial T1 mapping using a radial MOLLI sequence with FLASH readout. Magnetic Resonance in Medicine, Epub ahead of print.
  H. Reyngoudt, S. Turk and P.G. Carlier: 1H NMRS of carnosine combined with 31P NMRS to better characterize skeletal muscle pH dysregulation in Duchenne muscular dystrophy. NMR in Biomedicine, 2018, 31(1).
• J.L. Thibaud, B. Matot, I. Barthelemy, Y. Fromes, S. Blot and P.G. Carlier: Anatomical and mesoscopic characterization of the dystrophic diaphragm: An in vivo nuclear magnetic resonance imaging study in the Golden retriever muscular dystrophy dog. Neuromuscular Disorders, 2017, 27(4): 315-325.
• C. Le Guiner, L. Servais, M. Montus, T. Larcher, B. Fraysse, S. Moullec, M. Allais, V. Francois, M. Dutilleul, A. Malerba, T. Koo, J.L. Thibaut, B. Matot, M. Devaux, J. Le Duff, J.Y. Deschamps, I. Barthelemy, S. Blot, I. Testault, K. Wahbi, S. Ederhy, S. Martin, P. Veron, C. Georger, T. Athanasopoulos, C. Masurier, F. Mingozzi, P. Carlier, B. Gjata, J.Y. Hogrel, O. Adjali, F. Mavilio, T. Voit, P. Moullier and G. Dickson: Long-term microdystrophin gene therapy is effective in a canine model of Duchenne muscular dystrophy. Nature communications, 2017, 8: 16105.
• E.C.A. Araujo, N. Azzabou, A. Vignaud, G. Guillot and P.G. Carlier: Quantitative ultrashort TE imaging of the short-T2 components in skeletal muscle using an extended echo-subtraction method. Magnetic Resonance in Medicine, 2017, 78(3): 997-1008.