Mode non-voyant cliquez ici
 

Projects

Stem Cell Regulators

The overall aims of our research project are:
  • Generation of PW1 murine genetic models
  • Characterization of the « interstitial » PW1+ cells in post-natal muscle: are they a previously unrecognized stem cell population?
  • Identification of PW1 protein partners
  • Analysis of the stress response pathway in a progeria mouse model
  • Myotube stress responses: nuclear signals in pathological models
  • The ‘seed or soil’ hypothesis revisited-why is skeletal muscle rarely a site of 2o tumour metastases?
  • Mesoangioblasts and their regulation of stem cell capacity
  • Identification and characterization of novel stem cell niches
  • Identification of novel stem cell mobilizing agents
Stem cell fusing
Stem cell fusing - A 'green labelled' (GFP) stem cell (see arrow) fusing with forming myotubes from satellite cells. In 4 hours, the green cell converts from a stem cell into muscle when place in culture. This novel population of cells are called PICs which we have identified in the lab.
Representative Publications:
  1. Mitchell, K.J., A. Pannerec, B. Cadot, A. Parlakian, V. Besson, E.R. Gomes, G. Marazzi, and D.A. Sassoon, Identification and characterization of a non-satellite cell muscle resident progenitor during postnatal development. Nat Cell Biol, 2010 Mar;12(3):257-66.
  2. Schwarzkopf, M., D. Coletti, G. Marazzi, and D. Sassoon, Chronic p53 activity leads to skeletal muscle atrophy and muscle stem cell perturbation. Basic Applied Myology, 2008. in press.
  3. Moresi, V., A. Pristera, B.M. Scicchitano, M. Molinaro, L. Teodori, D. Sassoon, S. Adamo, and D. Coletti, Tumor necrosis factor-alpha inhibition of skeletal muscle regeneration is mediated by a caspase-dependent stem cell response. Stem Cells, 2008. 26(4): p. 997-1008.
  4. Schwarzkopf, M., D. Coletti, D. Sassoon, and G. Marazzi, Muscle cachexia is regulated by a p53-PW1/Peg3-dependent pathway. Genes Dev, 2006. 20(24): p. 3440-52.
     
  5. Nicolas, N., G. Marazzi, K. Kelley, and D. Sassoon, Embryonic deregulation of muscle stress signaling pathways leads to altered postnatal stem cell behavior and a failure in postnatal muscle growth. Dev Biol, 2005. 281(2): p. 171-83. 
  6. Polekhina, G., C.M. House, N. Traficante, J.P. Mackay, F. Relaix, D.A. Sassoon, M.W. Parker, and D.D. Bowtell, Siah ubiquitin ligase is structurally related to TRAF and modulates TNF-alpha signaling. Nat Struct Biol, 2002. 9(1): p. 68-75.
  7. Relaix, F., X. Wei, W. Li, J. Pan, Y. Lin, D.D. Bowtell, D.A. Sassoon, and X. Wu, Pw1/Peg3 is a potential cell death mediator and cooperates with Siah1a in p53-mediated apoptosis. Proc Natl Acad Sci U S A, 2000. 97(5): p. 2105-10.
  8. Relaix, F., X.J. Wei, X. Wu, and D.A. Sassoon, Peg3/Pw1 is an imprinted gene involved in the TNF-NFkappaB signal transduction pathway. Nat Genet, 1998. 18(3): p. 287-91.
 
Update: July 2010
 
Institut de Myologie - 47/83 Bd de l'hôpital - 75013 Paris - Tél. : +33 1 42 16 58 58